Cell Line: A-498

Cell Description: Human kidney carcinoma

Culture medium: Minimum essential medium (Eagle) in Earle's BSS with sodium bicarbonate, 2 mM L-glutamine and 1,0 mM sodium pyruvate, 1% nonessential amino acids, 90%; fetal bovine serum 10%; G6PD, B

Subculture routine: Remove medium, rinse with fresh 0.025% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37°C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.

Split Ratio: A ratio of 1:2 to 1:4 is recommended

Morphology: epithelial

Karyotype: 2n = 46

Comments: Derived from a 52 years old female.

Sterility: Tests for Mycoplasma, bacteria and fungi were negative

Biosafety Level: 1

Reference: Fogh J et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214,1977. J. Natl. Cancer Inst. 59: 221-226, 1977. Faust JB and Meeker TC. Amplification and expression of the bcl-1 gene in human solid tumor cell lines. Cancer Res. 52: 2460-2463, 1992. Giard DJ et al. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. J.Natl. Cancer Inst. 51: 1417-1423, 1973. Natl. Cancer Inst. Monogr. 49: 5-9, 1978.