Cell Line: AGS

Cell Description: Human gastric adenocarcinoma; stomach

Culture Medium: Ham's F12 medium with sodium bicarbonate, 2 mM L-glutamine and 1,0 mM sodium pyruvate, 90%; fetal bovine serum, 10%

Subculturing: Remove medium, rinse monolayer with fresh 0.025% trypsin, 0.025% EDTA solution in PBS, remove trypsin and let the culture incubate at 37°C for 3 to 5 minutes. Add fresh medium, aspirate and dispense into new flasks.

Split Ratio: A ratio of 1:2 to 1:6 is recommended

Description: The AGS cell line was derived from fragments of a tumor resected from a patient who had received no prior therapy. The cells have a plating efficiency of 34% in the medium below.

Morphology: epithelial

Growth Properties: monolayer

Doubling Time: 20

Sterility: Tests for Mycoplasma, bacteria and fungi were negative

Biosafety Level: 1

Tumorigenic: yes, in athymic BALB/c mice

Karyotype: modal number = 47; range = 39 to 92

Comments: Derived from a 54 year old caucasian female

Depositor: Barranco

References: Barranco SC et al. Heterogeneous responses of an in vitro model of human stomach cancer to anticancer drugs. Invest. New Drugs 1: 117-127, 1983. Barranco SC et al. Establishment and characterization of an in vitro model system for human adenocarcinoma of the stomach. Cancer Res. 43: 1703-1709, 1983. Rieder G et al. Role of adherence in Interleukin-8 induction in Helicobacter pylori-associated gastritis. Infect. Immun. 65: 3622-3630, 1997. Segal ED et al. Helicobacter pylori attachment to gastric cells induces cytoskeletal rearrangements and tyrosine phosphorylation of host cell proteins. Proc. Natl. Acad. Sci. USA 93: 1259-1264, 1996.