Cell Line: Capan-2
Cell Description: Human
adenocarcinoma, pancreas
Culture
medium: Mc
Coy«s 5a medium with sodium bicarbonate, 2 mM L-glutamine and 1,0 mM sodium
pyruvate + 10% FBS
Subculture
routine: Split
confluent cultures 1:3 to 1: 6 using 0,25% trypsin or trypsin/EDTA; 5% CO2,
37¡C
Morphology: Polygonal
Grown as: Monolyer;
transferred 1:3 weekly
In
Vitro Cytopathology: (P8)
Adenocarcinoma
Nude
mouse: Produces
well-differentiated adenocarcinoma consistent with pancreatic carcinoma
Description: The
cells produce high levels of MUC-1 mucin mRNA, low levels of MUC-2 mRNA but do
not express the MUC-3 gene.
Comments: J.
Fogh isolated this line in 1975 and characterized the progeny as indicated
beloww. Patient Data: Age-56, male, Race-Caucasian; Blood Type-B+.
Species: Confirmed
as human by isoenzyme analysis.
Isoenzymes: Me-2,
2; PGM3, 2; PGM1, 1; ES-D, 1; AK-1, 1; G6PD, B; GLO-1, 2; Phenotype Frequency
Product: 0.0004
Blood
Group Antien: Confirmed
as B on cultured Colls
Depositor: Fogh
Sterility: Tests
for Mycoplasma, bacteria and fungi were negative
Biosafety
Level: 1
References: Dahiya
R et al. Mucin synthesis and secretion in various human epithelial cancer cell
lines that express the MUC-1 mucin gene. Cancer Res. 53: 1437-1443, 1993.
ATCC
designation: HTB
80