Cell Line: CCRF CEM

Cell Description: Human leukemia, acute lymphoblastic; blood, peripheral; T lymphoblast

Culture Medium: RPMI 1640 medium with sodium bicarbonate, 4.5 g/L glucose,

2 mM L-glutamine, 10 mM HEPES, and 1.0 mM sodium pyruvate, 80%; FBS, 20%

Subculturing: Cultures can be maintained by addition or replacement of medium. Establish new cultures at 3 X 10⁵ viable cells/ml.

Fluid Renewal: add fresh medium (20 to 30% by volume) every 2 to 3 days depending on cell density

Morphology: lymphoblast

Growth Properties: monolayer/suspension

Tumorigenic: yes, in nude mice

Antigen Expression: CD3 B (37%), CD4 (50%), CD5 (95%), CD7 (77%)

Isoenzymes: G6PD, B

Reverse Transcriptase: negative

Sterility: Tests for mycoplasma, bacteria and fungi were negative

Biosafety Level: 1

ATCC number: CCL 119

References:

Cancer 18: 522-529, 1965. Sandstrom PA and Buttke TM. Autocrine production of

extracellular catalase prevents apoptosis of the human CEM T-cell line in serum-free medium. Proc. Natl. Acad. Sci. USA 90: 4708-4712, 1993. Adams RA. Formal discussion: the role of transplantation in the experimental investigation of human leukemia and lymphoma. Cancer Res. 27: 2479-2482, 1967. Uzman BG et al. Morphologic variations in human leukemic lymphoblasts (CCRF-CEM cells) after long-term culture and exposure to chemotherapeutic agents. A study with the electron microscope. Cancer 19: 1725-1742, 1966. Adams RA et al. Leukemia: serial transplantation of human leukemic lymphoblasts in the newborn Syrian hamster. Cancer Res. 27: 772-783, 1967. Miranda et al. Isolation of the human PC6 gene encoding the putative host protease for HIV-1 gp160 processing in CD4+ T lymphocytes. Proc. Natl. Acad. Sci. USA 93: 7695-7700, 1996.