CLS-145 / Medium: HamÕs F12 / Zoom: 200 x
Cell Line: CLS-145
Cell Description: Human
gastric papillary adenocarcinoma; stomach, pars cardiaca
Culture
medium: Ham's
F12 medium with sodium bicarbonate, 2 mM L-glutamine and 1,0 mM sodium pyruvate
90%; fetal bovine serum 10%
Subculture
routine: Remove
medium, rinse monolayer with fresh 0.025% trypsin, 0.05% EDTA solution in PBS,
remove trypsin and let the culture incubate at 37”C for 3 to 5 minutes. Add
fresh medium, aspirate and dispense into new flasks.
Split Ratio: A
ratio of 1:2 to 1:6 is recommended
Doubling Time: 36
Description: The
CLS-145 cell line was derived from fragments of a tumor resected from a patient
who had received no prior therapy. MAP-TEST negative, PAS positive
Morphology: epithelial
Tumorigenic: yes,
in nude mice (Rhythm 20-30 days)
Comments: Derived
from a 70 years old european female.
Sterility: Tests
for Mycoplasma, bacteria and fungi were negative
Biosafety
Level: 1
Depositor: CLS
References:
not published