Cell Line: DU 145

Cell Description: carcinoma; prostate; from metastatic site: brain

Culture medium: MEM Eagle and Earle´s BSS with sodium bicarbonate, 2 mM L-glutamine and 1,0 mM sodium pyruvate and 0,1mM non-essential amino acids; 90% + 10% FBS

Subculture routine: Remove medium, add fresh 0,25% trypsin solution for 1 minute, remove trypsin and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days.

Split ratio: 1:4 to 1:6 is recommended

Fluid renewal: 2 to 3 times weekly

Morphology: epithelial

Sterility: Tests for Mycoplasma, bacteria and fungi were negative

Biosafety Level: 1

Karyotype: (P75) hypotriploid to tetraploid with abnormalities

including breaks, dicentrics, minutes and large

telocentric marker

Isoenzymes: Me-2, 1-2; PGM3, 2;PGM1, 1;ES-D, 1;AK-1, 1;G6PD,

B; GLO-1, 2; Phenotype Fequency Product: 0.0041

AntigenExp: Blood Type 0; Rh+

Depositor: ATCC

Comments:

DU 145 was isolated by K.R. Stone et al from a lesion in the brain of a patient with metastatic carcinoma of the prostate and a 3 year history of lymphocytic leukemia. The line is not detectably hormone sensitive, is only weakly positive for acid phosphatease and isolated cells from colonies in soft agar. Ultrastructural analyses of both the cell line and original tumor revealed microvilli, tonofilaments and desmosomes. Many mitochondria, well developed Golgi and heterogenous lysosomes. The cells do not express prostate antigen.