Cell Line: HEK
293
Cell Description: Human
embryonal kidney
Culture
medium: EMEM
(EBSS) with sodium bicarbonate + 1% NEAA or DMEM, 2 mM L-glutamine, 1,0 mM
sodium pyruvate + 10% FBS (heat inactivated)
Subculture
routine: Split
confluent cultures 1:2 to 1:3 i.e. seeding at 3 x 104 to 5 x 104
cells/cm2 using 0,25% trypsin or trypsin / EDTA; 5% CO2,
37¡C. Cells detach at room temperature and may take several days to retach.
Morphology: Epithelial
Karyotype: 2n
= 46
Sterility: Tests
for Mycoplasma, bacteria and fungi were negative
Biosafety Level: 1
Depositor: Obtained
from ATCC
Comments: Derived
from human embryonal kidney cells transformed with sheared human adenovirus
type 5 (Ad5) DNA. The cells are sensitive to human adenoviruses and adenovirus
DNA. They can be used to isolate transformation defective host-range mutants of
Ad5 und for titrating human adenoviruses.
ECACC
number: 85120602
ATCC
designation: CRL
1573
Reference: J
Gen Virol, (1977), 36, 59; Virology, (1977), 77, 319