Cell Line: HeLa
Cell Description: Human
cervix carcinoma
Culture
medium: EMEM
(RBSS) with sodium bicarbonate + 1% NEAA,
or DMEM; 2 mM L-glutamine, 1 mM sodium pyruvate, + 10% FBS
Subculture
routine: Split
subconfluent cultures 1:3 to 1:10 i.e. seeding at 1x104 to
3x104 cells/cm2 using 0,25% trypsin or trypsin/EDTA; 5%
CO2
Description: The
cells are positive for keratin by immunoperoxidase staining. HeLa cells have
been reported to contain human papilloma virus 18 (HPV-18) sequences. P53 expression was
reported to be low, and normal levels of pRB (retinoblastoma suppressor) were
found.
Products: keratin
Lysophosphatidylcholine (lyso-PC) induces AP-1 activity and c-jun N-terminal
kinase activity (JNK1) by a protein kinase C-independent pathway
Isoenzymes: G6PD,
A
Morphology: Epithelial
Karyotype: 2n
= 46; aneuploid
Applications: transfection
host
Depositor: Prof.
J. B. Griffiths, PHLS CAMR, Porton Down, Salisbury
Sterility: Tests
for Mycoplasma, bacteria and fungi were negative
Comments: The
1st aneuploid, epithelial-like line derived from human tissue and maintained by
serial subculture. Identified as a contaminant in many subsequently derived
lines, due to poor culture techniques. Susceptible to poliovirus 1 and
adenovirus type 3. Derived from a 31 years old black female.
ECACC
number: 85060701
ATCC
designation: CCL
2
Reference: J.
Exp. Med. 1953; 97: 695