Cell Line: HL-60

Cell Description: Human leukemia, acute promyelocytic; blood, peripheral

Culture Medium: Iscove's modified Dulbecco's medium with sodium bicarbonate, 4 mM L-glutamine, 1,0 mM sodium pyruvate adjusted, 80%; fetal bovine serum, 20%

Subculturing: Cultures can be maintained by addition or replacement of medium. Start new cultures at 2 x 10⁵ viable cells/ml and subculture at 1 x 10⁶ cells/ml.

Fluid Renewal: every 2 to 3 days

Morphology: lymphoblast

Growth Properties: suspesnsion

Oncogene: myc +

Receptors Expressed: complement; Fc

Isoenzymes: G6PD, B; PGM1, 1; PGM3, 1; ES-D,1; Me-2, 1; AK-1, 1; GLO-1, 1

Products: tumor necrosis factor (TNF), also known as tumor necrosis factor alpha (TNF-alpha, TNF alpha), after stimulation with phorbol myristic acid

Depositor: Gallagher

Description: HL-60 cells spontaneously differentiate and differentiation can be stimulated by butyrate, hypoxanthine, phorbol myristic acid (PMA, TPA), dimethylsulfoxide (DMSO, 1% to 1.5%), actinomycin D, and retinoic acid. The cells exhibit phagocytic activity and responsiveness to chemotactic stimuli. The line is positive for myc-oncogene expression.

Sterility: Tests for Mycoplasma, bacteria and fungi were negative

Biosafety Level: 1

Reverse Transcriptase: negative

Comments: Established from a 36 year old Caucasian female

References:

Gallagher R et al. Characterization of the continuous, differentiating myeloid cell line (HL-60) from a patient with acute promyelocytic leukemia. Blood 54: 713-733, 1979. Collins SJ et al. Continuous growth and differentiation of human myeloid leukaemic cells in suspension culture. Nature 270:347-349, 1977. Mullineaux PM et al. Human tumor necrosis factor. Production,purification, and characterization. J. Biol. Chem. 260: 2345-2354, 1985. Nahm MH et al. Identification of cross-reactive antibodies with low opsonophogocytic activity for Streptoccus pneumoniae. J. Infect. Dis. 176: 698-703, 1997. Berninghausen O and Leippe M. Necrosis versus apoptosis as the mechanism of target cell death induced by Entamoeba histolytica. Infect. Immun. 65: 3615-3621, 1997. Aparicio CL et al. Correction for label leakage in fluorimetric assays of cell adhesion. Biotechniques 23: 1056-1060,1997. Mansat V et al. The protein kinase C ctivators phorbol esters and phosphatidylserine inhibit neutral phingomyelinase ctivation, ceramide generation, and apoptosis triggered by daunorubicin. Cancer Res. 57: 5300-5304, 1997. Cuthbert JA and Lipsky PE. Regulation of proliferation and Ras localization in transformed cells by products of mevalonate metabolism. Cancer Res. 57: 3498-3504, 1997. Michael JM et al. Resistance to radiation-induced apoptosis in Burkitt's lumphoma cells is associated with defective ceramide signaling. Cancer Res. 57: 3600-3605, 1997. Clark RA et al. Tenascin supports lymphocyte rolling. J. Cell Biol. 137: 755-765, 1997.Tiffany HL et al. Enhanced expression of the eosinophil-derived neurotoin ribonuclease (RNS2) gene requires interaction between the promoter and intron. J. Biol. Chem. 271: 12387-12393, 1996. Chan YJ et al. Synergistic interactions between overlapping binding sites for the serum response factor and ELK-1 proteins mediate both basal enhancement and phorbol ester responsiveness of primate cytomegalovirus. J. Virol. 70: 8590-8605, 1996. Mao M et al. RIG-E, a human homolog of the murine Ly-6 family, is induced by retinoic acid during the differentiation of acute promyelocytic leukemia cell. Proc. Natl. Acad. Sci. USA 93: 5910-5914, 1996. Lepley RA et al. Tyrosine kinase activity modulates catalysis and translocation of cellular 5-lipoxygenase. J. Biol. Chem. 271: 6179-6184, 1996. Chen HM et al. Octamer binding factors and their coactivator can activate the murine PU.1 (spi-1) promoter. J. Biol. Chem. 271: 15743-15752, 1996.