Cell Line: HT 1080

Cell Description: human fibrosarcoma (male, 35 years, Caucasian)

Culture medium: MEM Eagle with Earle`s BSS, sodium bicarbonate, 0,1 mM non essential amino acids, 2mM L-Glutamine and 1mM sodium pyruvate, 90%; heat-inactivated FBS, 10%

Subculture routine: Remove medium, add fresh 0,25% trypsin, 0,02% EDTA solution, rinse and remove. Allow flask to sit at room temperature (or 37°C) until cells detach (approximately 10 min). Add fresh medium, aspirate and dispense into new flasks. Split confluent cultures 1:4 to 1: 8

Morphology: Epithelial

Virus Suscept: poliovirus 1; vesicular stomatitis (Indiana); RD114; feline leukemia virus (FeLV)

Tumorigenic: yes, in immunosuppressed mice

Oncogene: ras +

Transcriptase: negative

Isoenzymes: G6PD, B

Sterility: Tests for Mycoplasma, bacteria and fungi were negative

Biosafety Level: 1

Comments: The cells contain an activated N-ras oncogene.

ATCC number: CCL-121

References:

Chen TR et al. Intercellular karyotypic similarity in near-diploid cell lines of human tumor origins. Cancer Genet. Cytogenet. 10: 351, 1983. Geiser AG et al. Suppression of tumorigenicity in human cell hybrids derived from cell lines expressing different activated ras oncogenes. Cancer Res. 49: 1572-1577, 1989. Rasheed S et al. Characterization of a newly derived human sarcoma cell line (HAT-1080). Cancer 33: 1027-1033,1974. Adams RA et al. Direct implantation and serial transplantation of human acute lymphoblastic leukemia in hamsters, SB-2. Cancer Res. 28: 1121-, 1968. Proc Am Assic. Cancer Res. 8: 1, 1967. Hu M et al. Purification and characterisation of human lung fibroblast motility-stimulating factor for human soft tissue sarcoma cells: identification as an NH2-terminal fragment of human fibrinectin. Cancer Res. 57: 3577-3584,1997. Iida A et al. Inducible gene expression by retrovirus-mediated transfer of a modified tetracycline-regulated system. J. Virol. 70: 6054-6059, 1996. Brennemann M et al. Stimulation of intracromosomal homologous recombination in human cells by electroporation with site-specific endonucleases. Proc. Natl. Acad. Sci USA 93: 3608-3612, 1996. Seiffert D. Hydrolysis of platelet vitronectin by calpain. J. Biol. Chem. 271: 11170-11176,1996. Hocking AM et al. Eukaryotic expression of recombinant biglycan. J. Biol. Chem. 271: 19571-19577, 1996