Cell Line: HT-29

Cell Description: Human colon adenocarcinoma grade II

Culture medium: Mc Coy´s 5a with sodium bicarbonate, 2mM L-glutamine, and 1.0mM sodium pyruvate, 90%; FBS 10%

Subculture routine: Split confluent cultures 1:3 to 1: 10 i.e. seeding at 1 x 10⁴ to 3 x 10⁴ cells/cm² using 0,25%; 5% CO₂, 37°C

Morphology: Epithelial

Karyotype: 2n = 46; hypertriploid

Cell product: Secretory component of IgA, CEA

Sterility: Tests for Mycoplasma, bacteria and fungi were negative

Biosafety Level: 1

Comments: Cells isolated from a primary tumor in a 44 year old Caucasian female.

Applications: Tumourigenicity studies

ECACC number: 91072201

Reference: Human Tumor Cells in Vitro, 1975:225. Plenum Press, NY.