Cell Line: HuT 78

Cell Description: human embryo lymphoblast

Culture medium: RPMI 1640 with sodium bicarbonate, 2 mM L-glutamine and 1,0 mM sodium pyruvate + 10% FBS

Subculture routine: Maintain cultures between 3 x 10⁵ to 9 x 10⁵; 5% CO₂, 37°C

Morphology: Lymphoblast

Sterility: Tests for Mycoplasma, bacteria and fungi were negative

Biosafety Level: 1

Depositor: DR. G. Farrar, PHLS CAMR, Porton Down, Salisbury

Comments: Derived from peripheral blood of patient with Sezary syndrome. Cells exhibit the features of a nature T-cell line with inducer/helper phenotype. Cells produce IL-2 and express IL-2-receptors alpha IL2RA / beta IL2RB / gamma IL2RG.

ATCC number: TIB-161

Reference: J. Exp. Med. 1981; 154:1403; J. Exp. Med. 1959; 109:271; J. Biol. Chem, 1959; 234:1042

Subculture routine: Maintain cultures between 3 x 105 to 9 x 105; 5% CO2, 37°C

Morphology: Lymphoblast

Subculture routine: Maintain cultures between 3 x 10⁵ to 9 x 10⁵; 5% CO₂, 37°C