Cell Line: JEG-3

Cell Description: Human choriocarcinoma; placenta

Culture medium: MEM (Eagle) in Earles´s BSS with sodium bicarbonate, 2 mM L-glutamine and 1,0 mM sodium pyruvate, 1% non essential amino acids (NEAA), 10% fetal bovine serum.

Subculture routine: Remove medium, rinse with fresh 0,25% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37°C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days.

Morphology: Epithelial

Growth Properties: Monolayer

Split Ratio: 1:4 to 1:6 is recommended

Sterility: Tests for Mycoplasma, bacteria and fungi were negative

Level: 1

Tumorigenic: yes, in nude mice; forms malignant tumor consistent with choriocarcinoma.

Isoenzymes: PGM3, 1-2; PGM1, 1; ES-D, 1; GLO-1, 1-2; G6PD, B

Products: human chorionic gonadotropin (hCG), human chorionic somatomammotropin (placental lactogen); progesterone; able to transform steroid precursors to estrone and estradiol.

Comments: This is one of six clonally derived lines isolated from the Woods strain of the Erwin-Turner tumor by Kohler and associates.

ATCC designation: HTB 36

Reference:

Kohler PO and Bridson WE. Isolation of hormone-producing clonal lines of human choriocarcinoma. J. Clin. Endocrinol. 32: 683-687, 1971. Fogh J et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. J. Natl. Cancer Inst. 59: 221-226, 1977 Acta Endocrinol. Suppl. 153: 137-153, 1971. Landers JE et al. Translational enhancement of mdm2 oncogene expression in human tumor cells containing a stabilized wild-type p53 protein: Cancer Res. 57: 3562-3568, 1997. Foesler WJ et al. The alpha-isoform of the CCAAT/enhancer-binding protein is required for mediating cAMP responsiveness of the phosphoenolpyruvate carboxykinase promoter in hepatoma cells. J. Biol. Chem. 271: 8068-8074, 1996.