Cell Line: Jurkat E6-1

Cell Description: Acute T cell leukemia, human

Culture medium: RPMI 1640 with sodium bicarbonate, 2 mM L-glutamine and 1,0 mM sodium pyruvate + 10% FBS

Comments: This clone of the Jurkat-FHCRC cell line produces large amounts of interleukin-2 (IL-2) is produced with phorbol esters and either lectins of monoclonal antibodies against the T3 T cell antigen receptor. No IL-2 is produced in the absence of stimuli, and both stimuli are required for activation and lymphokine production. The line was cloned from Jurkat-FHCRC cells obtained from K. Smith and have tested negative for mycoplasma. The cell can also be induced to produce human g interferon.

Sterility: Tests for Mycoplasma, bacteria and fungi were negative

Biosafety Level: 1

ATCC designation: TIB 152

Submitted by: A. Weiss, Howard Hughes Medical Institute, University of California, San Francisco, CA

Reference: J. Immunol. 133:123-128, 1984