Cell Line: MOLT-4
Cell Description: Leukemia, acute lymphoblastic; T lymphoblast
Culture
medium: RPMI
1640 with 4,5 g/L glucose, sodium bicarbonate, 2 mM L-glutamine and 1,0 mM
sodium pyruvate, 10mM HEPES, 90%; FBS, 10%
Subculture
routine: Cultures
can be maintained by addition or replacement of fresh medium. Start new
cultures at 4 x 105 cells/ml and subculture before the cell density
reaches 2 x 106 cells/ml
Morphology: Lymphoblast
AntigenExp: CD1
(49%), CD2 (35%), CD3 A (26%) B (33%) C (34%), CD4 (55%), CD5 (72%), CD6 (22%),
CD7 (77%)
Products: High levels of terminal deoxynucleotidyl transferase (TdT) are produced
Depositor: Nowada
Comments: The
cells do not produce immunoglobulin or Epstein-Barr virus. There is a G-> A
mutation at codon 248 of the p53 gene. P53 is not expressed. The patient had
received prior multidrug chemotherapy. MOLT-4 was derived from the same patient
as the MOLT-3 cell line (ATCC: CRL 1552).
ATCC
designation: CRL
1582
Reference: Sandstrom
PA and Buttke TM. Autocrine production of extracellular catalase prevents
apoptosis of the human CEM T-cell line in serum-free medium. Proc. Natl. Acad.
Sci. USA 90: 4708-4712, 1993