Cell Line: Reh

Cell Description: Human leukaemia, acute lymphocytic (non-T; non-B)

Culture medium: RPMI 1640 with sodium bicarbonate, 2 mM L-glutamine, 1,0 mM sodium pyruvate + 10% FBS

Subculture routine: Cultures can be maintained by addition or replacement

of medium. Start cultures at 2x 10⁶ cells/ml and

maintain between 1 x 10⁵ and 1 x 10⁶ cells/ml.

Incubate at 5 % CO₂, 37°C

Morphology: Lymphoblast

Growth properties: Suspension

Anigen Exp: CD3 A (17%) B(17%) C(20%), CD4(15%),

CD10 (55%)

Sterility: Tests for Mycoplasma, bacteria and fungi were negative

Biosafety Level: 1

Reference: Biochem. Biophys. Acta 516: 192-230, 1978

ATCC designation: CRL 8286