Cell Line: RPMI
1788
Cell Description: Human peripheral blood, B lymphocyte, EBNA positive
Culture
medium: RPMI
1640 with sodium bicarbonate, 2 mM L-glutamine, 1,0 mM sodium pyruvate + 20%
FBS
Subculture
routine: Maintain
culutres between 3 Ð 4 x 105 Cells/ml; Maximum cell density 1 Ð 2 x
106 cells/ml.
Incubate at 5% CO2,
37¡C
Morphology: Lymphoblast
Karyotype: 2n
= 46
Cell product: IgM
(Lambda light chain)
Histocompatibility type: A2,
Aw33, B7, B14
Depositor: Obtained
from ATCC
Comments: Derived
from the peripheral blood of an apparently normal 33 year old Caucasian male.
The cells secrete IgM (lambda light chain). Handle as a potential biohazard
under at least containment level 2.
Sterility: Tests
for Mycoplasma, bacteria and fungi were negative
Biosafety
Level: 2
ECACC
number: 85112106
ATCC
designation: CCL
156
Reference: J.
Nat. Cancer Inst. 1969; 43:1119