Cell Line: SK-BR-3

Cell Description: Human; pleural effusion (metastasis); adenocarcinoma

(colon primary)

Culture medium: McCoy's 5a medium with sodium bicarbonate, 2 mM L-glutamine and 1,0 mM sodium pyruvate, 90%; fetal bovine serum, 10%

Subculture routine: Remove medium, rinse with fresh 0.025% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37°C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.

Split Ratio: A ratio of 1:2 to 1:4 is recommended

Morphology: Epithelial

Karyotype: (P9) hypertriploid to hypotetraploid (+A, +B, +C, +E, +F, +G, -D) with abnormalities including dicentrics, acrocentric fragments, rings, secondary constrictions, large metacentrics or polycentrics and large submetacentric marker

AntigenExp: Blood Type A; Rh+; HLA A11, Bw22(+/-), B40, B18

Isoenzymes: PGM3, 1; PGM1, 1-2; ES-D, 1; AK-1, 1-2; GLO-1, 2; G6PD, B; Phenotype Frequency Product: 0.0044

Tumorigenic: yes, in nude mice; forms poorly differentiated adenocarcinoma

Depositor: Trempe

Comments: Established from 43-year-old female Caucasian

Sterility: Tests for Mycoplasma, bacteria and fungi were negative

Biosafety Level: 1

ATCC designation: HTB-30

Reference: Fogh, J., ed., Human tumor cells in vitro. New York: Plenum

Press; 1975:pp. 115-159.Trempe GL. Human breast cancer in culture. Recent Results Cancer Res.: 33-341, 1976. Fogh J et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214,1977. J. Natl. Cancer Inst. 59: 221-226, 1977. Pollack MS et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J. Natl. Cancer Inst. 66: 1003-1012, 1981. Littlewood-Evans AJ et al. The osteoclast-associated protease cathepsin K is expressed in human breast carcinoma. Cancer Res. 57: 5386-5390, 1997. Chavany C et al. p185erbB2 binds to GRP94 in vivo. J. Biol. Chem. 271: 4974-4977, 1996.