Cell Line: SK-LMS-1

Cell Description: Human leiomyosarcoma; vulva

Culture medium: MEM (Eagle) in Earle´s BSS with sodium bicarbonate, 2 mM L-glutamine, 1,0 mM sodium pyruvate, 0,1mM non-essential amino acids + 10% FBS

Subculture routine: Remove medium, add fresh 0,25% trypsin solution for 3 to 5 minutes, remove trypsin , add fresh medium, aspirate and dispens into new flasks. Subculture every 6 to 8 days

Split Ratio: 1:2 to 1:5 is recommended

Morphology: Fibroblast

Karyotype: (P12) hypotriploid to hypertriploid (+A2, +A3, +C, +D, +E, +F, +G, -A) with abnormalities including dicentrics, acrocentric fragments, breaks, secondary constrictions, minutes and large submetacentric markers.

AntigenExp: Blood Type 0; Rh+

Isoenzymes: Mw-2, 2; PGM3, 1-2; PGM1, 1-2;ES-D, 1; AK-1; GLO-1, 1-2; G6PD, B;

Phenotype frequency Prod.: 0.0027

Tumorigenic: yes, in nude mice; forms leiomyosarcoma

Depositor: Old

Comments: Established from 43-year-old female Caucasian

Sterility: Tests for Mycoplasma, bacteria and fungi were negative

Biosafety Level: 1

ATCC designation: HTB 88

Reference: Fogh, J., ed., Human tumor cells in vitro. New Yourk: Plenum Press; 1975:pp. 115-159. Fogh J et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. Goodfellow M et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. Koochekpour S et al. Met and hepatocyte growtz factro/scatter factor expression in human gliomas. Cancer Res. 57: 5391-5398, 1997. Hu M et al. Purification and characterization of human lung firbrblast motility-stimulating factor for human soft tissue sarcoma cells: identification as an NH2-terminal fragment of human fibronectin. Cancer Res. 57: 3577-3584, 1997