Cell Line: SK-MEL-28

Cell Description: Human malignant melanoma; skin

Culture medium: MEM (Eagle) with Hanks´ BSS, sodium bicarbonate, 2 mM L-glutamine, 1,0 mM sodium pyruvate and 0,1mM non essential amino acids + 10% FBS

Subculture routine: Remove medium, add fresh 0,25% trypsin solution for 2 to 3 minutes, remove trypsin, add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days.

Morphology: Polygonal

Growth Properties: Monolayer

Split ratio: 1:2 to 1:5 is recommended

Isoenzymes: PGM1, 1; PGM3, 1; ES-D, 1; AK-1, 1-2; GLO-1, 2; G6PD, B

AnigenExp: Blood Type A; Rh+; HLA A11, A26, B40, Drw4

Tumorigenic: yes, in nude mice; forms malignant melanoma (large round cell type)

Depositor: T. Takahashi

Comments: This is one of a very extensive series of melanoma lines (see also ATCC HTB 70, AtCC HTB 71 and ATCC HTB 73) isolated by T. Takahashi and associates.

Sterility: Tests for Mycoplasma, bacteria and fungi were negative

Biosafety Level: 1

ATCC designation: HTB 72

References: RF32972: J. Natl. Cancer Inst. 59: 221-226, 1977 PubMed: 77210034

RF33620: Pollack MS et al. HLA-A, B, C and DR alloantigen expression on 46 cultured human tumor cell lines. J. Natl. Cancer Inst. 66: 1003-1012, 1981 PubMed: 81218998 RF33649: Carey TE et al. Cell surface antigens of human malignant melanoma: mixed hemadsorption assays for humoral immunity to cultured autologous melanoma dells. Proc. Natl. Acad. Sci. USA 73: 3278-3282, 1976 PubMed: 77013525