Cell Line: SK-MEL-5

Cell Description: Human malignant melanoma, metastastatic site: acillary node

Culture medium: MEM (Eagle) with Earle´s BSS, sodium bicarbonate, 2 mM L-glutamine, 1,0 mM sodium pyruvate, 0,1mM non-essential amino acids + 10% FBS

Subculture routine: Remove medium, add fresh 0,25% trypsin solution for 1 minute, remove trypsin and let the culutre sit at room termperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days.

Morphology: Stellate

Growth Properties: Monolayer

Isoenzymes: PGM1, 1-2; PGM3, 1; ES-D, 1; AK-1, 1; GLO-1, 1-2; G6PD, B;

Phenotype Frequency Prod.: 0.0860

AnigenExp: Blood Type 0; Rh+; HLA A2, A11, B40, Bw16

Tumorigenic: yes, in nude mice; forms malignant melanoma

Depositor: T. Takahashi

Comments: This is one of a very extensive series of melanoma lines (see also ATCC HTB 71, AtCC HTB 72 and ATCC HTB 73) isolated by T. Takahashi and associates. The lines served as source of target cells for detection of melanoma specific antibody in patients with the disease.

Sterility: Tests for mycoplasma, bacteria and fungi were negative

Biosafety Level: 1

ATCC designation: HTB 70

References: RF32696: Fogh J et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977 PubMed: 77097006 RF32972: J. Natl. Cancer Inst. 59: 221-226, 1977 PubMed: 77210034 RF33620: Pollack MS et al. HLA-A, B, C and DR alloantigen expression on 46 cultured human tumor cell lines. J. Natl. Cancer Inst. 66: 1003-1012, 1981 PubMed: 81218998 RF33649: Carey TE et al. Cell surface antigens of human malignant melanoma: mixed hemadsorption assays for humoral immunity to cultured autologous melanoma dells. Proc. Natl. Acad. Sci. USA 73: 3278-3282, 1976 PubMed: 77013525