Cell Line: SK-N-LO

Cell Description: Human neuroblastoma

Culture medium: MEM (Eagle) with Earle´s BSS, sodium bicarbonate, 2mM L-glutamine, 1.0mM sodium pyruvate, 0,1mM non-essential amino acids + 10% FBS

Subculture routine: Remove medium, rinse with fresh 0,25% trypsin solution and let the culutre sit at room termperature (or at 37°C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.

Subculture: every 6 to 8 days.

Split ratio: 1:6 to 1:12 is recommended

Morphology: Epithelial

Growth Properties: Monolayer

Depositor: Buck

Sterility: Tests for Mycoplasma, bacteria and fungi were negative

Biosafety Level: 1

References: Cancer Res. 1988 Jun 1; 48(119: 2993-7; Anticancer Res. 1994 Jan-Feb; 14(1A):221-7; Melanoma Res. 1994 Apr; 4(2): 101-6; Anticancer Res. 1994 Sep-Oct; 14 (5A): 1903-6; Anticancer Res. 1997 Mar-Apr; 17(2A): 981-6; Anticancer Res. 1997 Jul-Aug; 17(4B): 3177-8.