Cell Line: SK-N-MC
Cell
Description: Human
neuroepithelioma; brain; from metastatic site: supraorbitalarea
Culture
medium: MEM
(Eagle) with Earle«s BSS, sodium bicarbonate, 2mM L-glutamine, 1.0 mM sodium
pyruvate, 0,1 mM non-essential amino acids + 10% FBS
Subculture
routine: Remove
medium, rinse with fresh 0,25% trypsin solution, remove trypsin and let the
culture sit at room temperature (or at 37¡C) until the cells detach (about 10
minutes). Add fresh medium, aspirate
and dispense into new flasks.
Subculture: every
6 to 8 days
Split Ratio: 1:6
to 1:12 is recommended
Morphology: Epithelial
Karyotype: Hypodiploidy
to pseudodiploidy. Abnormalities including double minutes. breaks; large
submetacentric, telocentric and small telocentric markers (originator). (P32)
Hypodiploid to hyperdiploid and triploid to hypotetraploid with abnormalities
including dicentrics, breaks, double minutes (DM), large subtelocentric and
small telocentric chromosomes.
Phenotype
Frequency Prod.: 0.00005
AntigenExp: Blood
Type 0; Rh+
Isoenzymes: Me-2,
2; PGM3, 1-2; PGM1, 1-2;ES-D, 2; AK-1, 1; GLO-1, 1-2; G6PD, B;
Tumorigenic: yes,
in nude mice; also in hamster cheek
Comments: This
is one of two cell lines (see ATCC HTB-11) of neurogenic origin derived by J.
L. Biedler. SK-N-MC was isolated in September of 1971 and was found to have
moderate dopamine Ð beta Ð hydroxylase acivity as well as formaldehyde induced
fluorescence indicative of intracellular catecholamines.
Sterility: Tests
for Mycoplasma, bacteria and fungi were negative
Biosafety
Level: 1
Reference: Spengler
BA et al. Morphology and growth, tumorigenicity, and cytogenetics of human
neuroblastoma cells established in vitro. In Vitro 8: 410, 1973.
ATCC
designation: HTB
10