Cell Line: SK-OV-3

Cell Description: Human adenocarcinoma; ovary; ascites

Culture medium: McCoy's 5a medium with sodium bicarbonate, 2mM L-glutamine, 1.0mM sodium pyruvate + 10% fetal bovine serum. (Alternatively, DMEM:F-12 may be used)

Subculture routine: Remove medium, rinse with fresh 0.025% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37°C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.

Split Ratio: A ratio of 1:2 to 1:3 is recommended

Morphology: epithelial

Karyotype: (P16) hypodiploid to hypotetraploid with dicentrics and large telocentric

AntigenExp: Blood Type B; Rh+

Isoenzymes: PGM3, 1; PGM1, 1-2; ES-D, 1; Me-2, 1; AK-1, 1; GLO-1, 1-2; G6PD, B; Phenotype Frequency Product: 0.0311

Tumorigenic: yes, in nude mice; forms moderately well differentiated adenocarcinoma consistent with ovarian primary

Depositor: Trempe

Sterility: Tests for Mycoplasma, bacteria and fungi were negative

Biosafety Level: 1

Comments: Derived from a 64 years old caucasian femal

ATCC No.: HTB-77

Reference:

Fogh, J. , ed., Human tumor cells in vitro. New York: Plenum Press; 1975:pp. 115-159. Fogh J et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. J. Natl. Cancer Inst. 59: 221-226, 1977. Morimoto H et al. Overcoming tumor necrosis factor and drug resistance of human tumor cell lines by combination treatment with anti-Fas antibody and drugs or toxins. Cancer Res. 53: 2591-2596, 1993. Morimoto H et al. Synergistic effect of tumor necrosis factor-alpha- and diphtheria toxin- ediated cytotoxicity in sensitive and resistant human ovarian tumor cell lines. J. Immunol. 147:2609-2616, 1991. Zhang X et al. Microfilament depletion and circumvention of multiple drug resistance by sphinxolides. Cancer Res. 57: 3751-3758,1997. Clinton GM et al. Estrogens increase the expression of fibulin-1, an extracellular matrix protein secreted by human ovarian cancer cells. Proc. Natl. Acad. Sci. USA 93: 316-320, 1996. Zhang X and Smith CD. Microtubule effects of welwistatin, a cyanobacterial indolinone that circumvents multiple drug resistance. Mol. Pharmacol. 49: 288-294, 1996.