Cell Line: SW1116

Cell Description: Human adenocarcinoma, colorectal; colon

Culture Medium: Leibowitz’s L-15 medium with sodium bicarbonate, 2mM L-glutamine, 1.0mM sodium pyruvate + 10% FBS

Subculturing: Remove medium, rinse the culture twice with fresh 0.025% trypsin, 0.02% EDTA, add fresh trypsin, EDTA and incubate for 10 to 20 minutes at 37°C. Add fresh medium, resuspend and centrifuge. Resuspend in fresh medium and dispense into new flasks.

Split Ratio: A ratio of 1:3 to 1:6 is recommended

Morphology: epithelial

Growth Properties: monolayer

Fluid Renewal: 1 to 2 times weekly

Sterility: Tests for Mycoplasma, bacteria and fungi were negative

Biosafety Level: 1

Tumorigenic: yes, in nude mice

Antigen Expression: blood type O; Rh+

Isoenzymes: G6PD, B; PGM1, 1; PGM3, 1-2; 6PGD, A; ES-D, 1;

PEP-D, 1

Reverse Transcriptase: negative

Products: carcinoembryonic antigen (CEA) 2654 ng/106 cells/10 days; keratin

Oncogene: myc +; myb + ; ras +; fos +; sis +; p53 +; abl -; ros -; src -

Depositor: A. Leibovitz

Description: Isolated from colorectal adenocarcinoma. The cells are CSAp negative (CSAp-) and colon antigen 3, negative. They are positive for keratin by immunoperoxidase staining. The line is positive for expression of c-myc, K-ras, H-ras, myb, sis and fos oncogenes. N-myc and N-ras expression were not detected. Tumor specific nuclear matrix proteins CC-4, CC-5 and CC-6 are expressed.

Comments: establised from a 73-year-old, grade III, Dukes´ type A, caucasian male

References:

Wright WC et al. Distinction of seventy-one cultured human tumor cell lines by polymorphic enzyme analysis. J. Natl. Cancer Inst. 66: 239-247, 1981. Leibovitz A et al. Classification of human colorectal adenocarcinoma cell lines. Cancer Res. 36: 4562-4569, 1976. Keesee SK et al. Nuclear matrix proteins in human colon cancer. Proc. Natl. Acad. Sci. USA 91: 1913-1916, 1994. Hao L. et al. PC-407 inhibited proliferation and induced apoptosis in human colon cancer SW-1116 cells. Acta Pharmacol Sin 25(11): 1509-1514, 2004.