Cell Line: SW-1353

Cell Description: human chondrosarcoma; bone

Culture Medium: Leibovitz's L-15 medium with sodium bicarbonate, 2 mM L-glutamine and 1,0 mM sodium pyruvate, 90%; FBS, 10%

Subculturing: Remove medium, rinse with fresh 0.025% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37°C) until the cells detach (about 10 minutes).Add fresh medium, aspirate and dispense into new flasks.

Morphology: fibroblast

Growth Properties: monolayer

Split Ratio: A ratio of 1:2 is recommended

Fluid Renewal: 2 to 3 times weekly

Sterility: Tests for Mycoplasma, bacteria and fungi were negative

Biosafety Level: 1

Karyotype: hyperdiploid 47, XX, +7; Except for the trisomic N7 no other chromosome markers are evident

Antigen Expression: Blood type O -

Isoenzymes: G6PD, B; PGM1, 1; PGM3, 2; ES-D, 2; AK-1, 1; GLO-1, 2 Phenotype Frequency Prod: 0.00009

Depositor: Leibovitz

Comments: established from a 72-year-old caucasian female; humerus

Description: The SW 1353 cell line was initiated by A. Leibovitz at the Scott and White Clinic, Temple, Texas in 1977 from a primary grade II chondrosarcoma of the right humerus obtained from a 72 year old female Caucasian. The initial culture medium was L-15 containing cortisone and insulin plus 10% fetal bovine serum and antibiotics.

ATCC No.: HTB-94

References:

Rodrigues M, Kalinowska W, Zielinski C, Sinzinger H. Verapamil decreases accumulation of 99Tcm-MIBI and 99Tcm-tetrofosmin in human breast cancer and soft tissue sarcoma cell lines. Nucl Med Commun. 2001 Jun;22(6):645-50. Rodriques M, Chehne F, Kalinowska W, Zielinski C, Sinzinger H. Comparative 99mTc-MIBI, 99mTc-tetrofosmin and 99mTc-furifosmin uptake in human soft tissue sarcoma cell lines. Eur J Nucl Med. 2000 Dec;27(12):1839-43. Zenmyo M, Komiya S, Hamada T, Hiraoka K, Suzuki R, Inoue A. p21 and parathyroid hormone-related peptide in the growth plate. Calcif Tissue Int. 2000 Nov;67(5):378-81. Merluzzi VJ, Faanes RB, Czajkowski M, Last-Barney K, Harrison PC, Kahn J, Rothlein R. Membrane-associated interleukin 1 activity on human U937 tumor cells: stimulation of PGE2 production by human chondrosarcoma cells. J Immunol. 1987 Jul 1;139(1):166-8.