Cell Line: THP-1

Cell Description: human acute monocytic leukemia, monocyte

Culture Medium: RPMI 1640 medium with 4.5 g/L glucose, sodium bicarbonate, 2mM L-glutamine, 1.0mM sodium pyruvate, 0.05mM 2-mercaptoethanol + 10-15% FBS (optional with 10 mM HEPES)

Subculturing: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10⁵ cells/ml and do not allow the cell concentration to exceed 1 x 10⁶ cells/ml.

Fluid Renewal: every 2 to 3 days

Growth Properties: suspension

Antigen Expression: HLA A2, A9, B5, DRw1, DRw2

Receptors Expressed: immunoglobulin (Fc); complement (C3b) Growth

Products: lysozyme

Depositor: CLS

Comments: established from a 1-year-old male

ATCC number: TIB-202

References: Tsuchiya S et al. Establishment and characterization of a human acute monocytic leukemia cell line (THP-1). Int. J. Cancer 26: 171-176, 1980; Glimcher LH et al. Induction of maturation in cultured human monocytic leukemia cells by a phorbol diester. Cancer Res. 42: 1530,1982 ; Skubitz KM et al. Human granulocyte surface molecules identified by murine monoclonal antibodies. J. Immunol. 131: 1882, 1983; Cuthbert JA and Lipsky PE. Regulation of proliferation and Ras localization in transformed cells by products of mevalonate metabolism. Cancer Res. 57: 3498-3504, 1997; Huang S et al. Adenovirus interaction with distinct integrins mediates separate events in cell entry and gene delivery to hematopoietic cells. J. Virol. 70: 4502-4508, 1996; Clark RA et al. Tenascin supports lymphocyte rolling. J. Cell Biol. 137: 755-765, 1997; Hambleton J et al. Activation of c-Jun N-terminal kinase in bacterial lipopolysaccharide-stimulated macrophages. Proc. Natl. Acad.Sci. USA 93: 2774-2778, 1996; Hsu HY et al. Inhibition of macrophage scavenger receptor activity by tumor necrosis factor-alpha is transcriptionally and post-transcriptionally regulated. J. Biol. Chem. 271: 7767-7773, 1996; Lucas M and Mazzone T. Cell surface proteoglycans modulate net synthesis and secretion of macrophage apolipoprotein E. J. Biol.Chem. 271: 13454-13460, 1996; Sando GN et al. Induction of ceramide glucosyltransferase activity in cultured human keratinocytes. J. Biol. Chem. 271: 22044-22051, 1996; Ollivier V et al. Elevated cyclic AMP inhibits NF-kappaB-mediated transcription in human monocytic cells and endothelial cells. J. Biol. Chem. 271: 20828-20835, 1996.