Cell Line: U-87 MG

Cell Description: human glioblastoma; astrocytoma; brain

Culture Medium: MEM Eagle with Earle's BSS, sodium bicarbonate, 2mM L-glutamine, 1.0mM sodium pyruvate, 0.1 mM non-essential amino acids + 10% FBS

Subculturing: Remove medium, rinse with fresh 0.025% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37°C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.

Morphology: epithelial

Growth Properties: monolayer

Split Ratio: A ratio of 1:2 to 1:5 is recommended

Fluid Renewal: 2 to 3 times weekly

Sterility: Tests for Mycoplasma, bacteria and fungi were negative

Biosafety Level: 1

Tumorigenic: yes, in nude mice

Karyotype: Chromosome Frequency Distribution 50 Cells: 2n = 46. The stemline chromosome number is hypodiploid, the 2S component occurring at 5.4%. The following nine markers were common to most S metaphases: t(1q;?3), t(1pl22q), t(6p;?11q-), (6q;?7p), t(7q;?), del(12q), t(20;1p;9q), t(?8p;?), and M1. Neither homogeneously staining regions (HSR) nor double minutes (DM) were detected; all S metaphases were monosomic for the X chromosome.

Antigen Expression: Blood Type A, Rh+

Isoenzymes: Me-2, 1; PGM3, 1; PGM1, 2; ES-D, 1; AK-1, 1; GLO-1, 1; G6PD, B; Phenotype Frequency Product: 0.0017

Depositor: Ponten

Description: This is one of a number of cell lines derived from malignant gliomas (see also ATCC HTB-15, ATCC HTB-16 and ATCC HTB-17) by J. Ponten and associates from 1966 to 1969.

Comments: established from a 44-year-old caucasian female, grade III

ATCC No.: HTB-14

References:

Beckman G et al. G-6-PD and PGM phenotypes of 16 continuous human tumor cell lines. Evidence against cross-contamination and contamination by HeLa cells. Hum. Hered. 21: 238, 1971. Fogh J et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. J. Natl. Cancer Inst. 59: 221-226, 1977. Cancer Res. 52: 2533-2529, 1992. Ponten J and Macintyre EH. Long term culture of normal and neoplastic human glia. Acta Pathol. Microbiol. Scand. 74: 465-486, 1968. Li YM et al. Molecular identity and cellular distribution of advanced glycation endproduct receptors: relationship of p60 to OST-48 and p90 to 80K-H membrane proteins. Proc. Natl. Acad. Sci. USA 93: 11047-11052, 1996.