Cell line: CCRF CEM
Cell description: Homo sapiens (human), leukemia, acute lymphoblastic; blood, peripheral; T lymphoblast
Patient Data: Age/Stage: 4 years, Gender: female, Ethnicity: Caucasian,
Morphology: lymphoblast
Tumorigenic: yes, in syrian hamsters
Reverse Transcriptase: negative Isoenzymes: G6PD, B AntigenExp: CD3 B (37%), CD4 (50%), CD5 (95%), CD7 (77%)
Fluid Renewal: Add fresh medium (20% to 30% by volume) every 2 to 3 days
Sub Culturing: Cultures can be maintained by addition or replacement of medium. Establish new cultures at 3 x 100.000 viable cells/ml.
Growth Properties: suspensionReferences:
Cancer 18: 522-529, 1965 Sandstrom PA and Buttke TM. Autocrine production of extracellular catalase prevents apoptosis of the human CEM T-cell line in serum-free medium.
Proc. Natl. Acad. Sci. USA 90: 4708-4712, 1993 Adams RA.: Formal discussion: the role of transplantation in the experimental investigation of human leukemia and lymphoma.
Cancer Res. 27: 2479-2482, 1967 Uzman B. G. et al.: Morphologic variations in human leukemic lymphoblasts (CCRF-CEM cells) after long-term culture and exposure to chemotherapeutic agents. A study with the electron microscope.
Cancer 19: 1725-1742, 1966 Adams R.A. et al.: Leukemia: serial transplantation of human leukemic lymphoblasts in the newborn Syrian hamster.
Cancer Res. 27: 772-783, 1967 Miranda L et al.: Isolation of the human PC6 gene encoding the putative host protease for HIV-1 gp160 processing in CD4+ T lymphocytes.
Proc. Natl. Acad. Sci. USA 93: 7695-7700, 1996 Propagation: Medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%ATCC Number: CCL-119