Cell name: Jurkat

Cell description: Human leukaemic T cell lymphoblast, derived from Jurkat FHCRC.
An IL-2 producing cell line, derived by incubating the cells at 41°C for 48h followed by a limiting dilution cloning over macrophages.
Morphology: Suspension
Medium: RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Sub Culture Routine: Maintain cultures between 3 - 9 x 100.000 cells/ml; 5% CO₂; 37°C.
Karyotype: Pseudodiploid, modal No 46
Products: Interleukin 2 (IL-2), human alpha interferon

Reference:
J Immunol 1984;133:123; J. Immunol. Meth. 1993;157:203-207

ECACC Number: 88042803

back to Collection of Human Somatic Cells