Cell name: PANC-1

Cell description: Homo sapiens (human); epithelioid carcinoma; pancreas;
Age/Stage: 56 years
Gender: male
Ethnicity: caucasian
Morphology: epithelial
Depositors: M. Lieber
Karyotype: modal number = 63; 3 marker chromosomes; 1 ring Isoenzymes: G6PD, B
Fluid Renewal: 2 to 3 times weekly
Sub Culturing: Remove medium, rinse with fresh 0.25% trypsin, 0.02% versene solution, withdraw and add 1 to 2 ml of trypsin, versene. Allow the culture to sit at room temperature or 37°C until cells are dispensed, add fresh medium, aspirate and dispense into new flasks.
Split Ratio: A ratio of 1:2 to 1:4 is recommended
Doubling Time: 52
Growth Properties: monolayer

Propagation: Medium: Dulbecco's modified Eagle's medium, 90%; fetal bovine serum, 10% Biosafety Level: 1
Comments: Growth is inhibited by 1 unit/ml L-asparaginase. The cells will grow in soft agar.

References:
Lieber M et al. Establishment of a continuous tumor-cell line (panc-1) from a human carcinoma of the exocrine pancreas. Int. J. Cancer 15: 741-747, 1975;
Wu MC et al. Mechanism of sensitivity of cultured pancreatic carcinoma to asparaginase. Int. J. Cancer 22: 728-733, 1978
Lan MS et al. Polypeptide core of a human pancreatic tumor mucin antigen. Cancer Res. 50: 2997-3001

ATCC Number: CRL-1469
ECACC Number: 87092802

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