Cell line: RT-4
Cell description: RT-4 are derived from a recurring papillary tumor of the urinary bladder of a 63 year old male caucasian (Blood Type O+).
Morphology: epithelial-like
Tumorigenic in cheek pouch of steroid treated hamsters.
Sterility: test for Mycoplasma, bacteria and fungi were negative
Karyotype: (P174) Hyperdiploid and hypertetraploid with abnormalities including dicentrics, breaks, translocations and minutes. Isoenzymes: Me-2, 1; PGM3, 1-2; ES D, 1-2; AK 1, 1; GLO-1, 1-2 ; G6PD, B.
Phenotype Frequency Product: 0,0050
HLA cell line Phenotype: A25(10), 3; B12; Cw3;
Propagation: Culture Medium: Mc Coy's 5a medium, 90%; fetal bovine serum, 10%
Subculture Routine: remove medium, rinse with 0,25% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37°C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
Subculture: every 6 to 8 days
Split Ratio: a ratio of 1:2 to 1:4 is recommended
Fluid Renewal: 2 to 3 times weekly
Growth Properties: monolayer
Biosafety Level 1
References: Webber, M.M.; Sekely, L.I. (eds): In vitro models for cancer research. Vol.IV, Boca Raton, FL: CRC Press; pp.103-125. O'Toole C. et al 1972: Cellular Immunity to human urinary bladder carcinoma. I. Correlation to clinical stage and radiotherapy. Int. J. Cancer 19: 77-91.(1972). Fogh et al. 1977: J. Nat. Cancer Inst. 58: 209-214. Lasfargues E Y et al.(1978): J. Nat. Cancer Inst. 61: 967-978. Lasfargues E Y et al (1979).: In Vitro 15: 61: 967-978. Rigby, CC & Franks LM (1970): Br. J. Cancer 24, 746-754. Pollak, MS et al (1981) : J. Natl. Cancer Inst. 66: 1003-1012.ATCC No: HTB-2