Cell line: SK-MEL-28

Cell line description: Homo sapiens (human), malignant melanoma; skin
Age/Stage: 51 years
Gender: male
Morphology: polygonal
Depositors: T. Takahashi
Tumorigenic: yes, in nude mice; forms malignant melanoma (large round cell type)
Isoenzymes: PGM3, 1; PGM1, 1; ES-D, 1; AK-1, 1-2; GLO-1, 2; G6PD, B Antigen Exp: Blood Type A; Rh+; HLA A11, A26, B40, DRw4
Freeze Medium: Culture medium, 95%; DMSO 5%
Fluid Renewal: 2 to 3 times weekly
Sub Culturing: Remove medium, add fresh 0.25% trypsin solution for 2 to 3 minutes, remove trypsin, add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days.
Split Ratio: A ratio of 1:2 to 1:5 is recommended
Growth Properties: monolayer

Propagation: Minimum essential medium (Eagle) in Hanks' BSS with 1 mM sodium pyruvate and nonessential amino acids, 90%; fetal bovine serum, 10%
Biosafety Level: 1

Comments: This is one of a very extensive series of melanoma lines (see also ATCC HTB 70,ATCC HTB-71 and ATCC HTB-73) isolated by T. Takahashi and associates.

References:
RF32972: J. Natl. Cancer Inst. 59: 221-226, 1977 PubMed: 77210034 RF33620: Pollack MS et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines.
J. Natl. Cancer Inst. 66: 1003-1012, 1981 PubMed: 81218998 RF33649: Carey TE et al. Cell surface antigens of human malignant melanoma: mixed hemadsorption assays for humoral immunity to cultured autologous melanoma cells. Proc. Natl. Acad. Sci. USA 73: 3278-3282, 1976 PubMed: 77013525

ATCC designation: HTB-72

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