Cell name: SK-N-MC

Cell description: Homo sapiens (human), neuroblastoma; brain; from metastatic site: supra-orbitalarea
Age/Stage: 14 years
Gender: female
Ethnicity: caucasian
Morphology: epithelial
Tumorigenic: yes, in nude mice and also in hamster cheek
Karyotype: hypodiploidy to pseudodiploidy. Abnormalities including double minutes, breaks; large submetacentric, telocentric and small telocentric markers (originator). (P32) Hypodiploid to hyperdiploid and triploid to hypotetraploid with abnormalities including dicentrics, breaks, double minutes (DM), large subtelocentric and small telocentric chromosomes.
Isoenzymes: Me-2, 2; PGM3, 1-2; PGM1, 1; ES-D, 2; AK-1, 1; GLO-1,1-2; G6PD, B;
Phenotype Frequency Product: 0.00005
Antigen Exp: Blood Type O; Rh+
Freeze Medium: Culture medium, FCS, 31%; DMSO, 10%
Fluid Renewal: 2 to 3 times per week
Sub Culturing: Remove medium, rinse with fresh 0.25% trypsin solution,remove trypsin and let the culture sit at room temperature (or at 37°C) until the cells detach (about 10 minutes).Add fresh medium, aspirate and dispense into new flasks.
Subculture every 6 to 8 days.
Split Ratio: A subcultivation ratio of 1:6 to 1:12 is recommended
Growth Properties: adherent

Comments: This is one of two cell lines (see ATCC HTB-11) of neurogenic origin derived by J.L. Biedler. SK-N-MC was isolated in September of 1971 and was found to have moderate dopamine - beta - hydroxylase activity as well as formaldehyde induced fluorescence indicative of intracellular catecholamines.

Propagation: Medium: Minimum essential medium Eagle with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%
Biosafety Level: 1

References:
Spengler BA et al. Morphology and growth, tumorigenicity, and cytogenetics of human neuroblastoma cells established in vitro. In Vitro 8: 410, 1973

ATCC Number: HTB-10

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